| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 531 | Methylocella silvestris | ATCCGCTTTGGCTCGACT | TTCGCCAGCGTCCTTCAA | 59.02 | 59.58 | 153 | 74 |
100.00%
|
100.00%
|
| Primer 532 | Methylocella silvestris | GATCCGCTTTGGCTCGACT | TTCGCCAGCGTCCTTCAA | 60.15 | 59.58 | 154 | 74 |
100.00%
|
100.00%
|
| Primer 533 | Methylocella silvestris | ACGGGTGCTTGTCATCCTG | AGACGCGATGGCCAAGAT | 60.00 | 59.10 | 299 | 74 |
100.00%
|
100.00%
|
| Primer 534 | Methylocella silvestris | TGCTGCTGCATGGCTTTC | AATGGCGTGCCGTCTGAT | 59.03 | 59.73 | 209 | 74 |
100.00%
|
100.00%
|
| Primer 535 | Methylocella silvestris | TGGAACAATCGACCGGACAA | GCAGCTGCTGAAACCGAAC | 59.61 | 60.08 | 163 | 74 |
100.00%
|
100.00%
|
| Primer 536 | Methylocella silvestris | TCGTCAAAGGCTCGATCGG | AACGCCTGAAGTCGCTGA | 59.86 | 59.27 | 252 | 74 |
100.00%
|
100.00%
|
| Primer 537 | Methylocella silvestris | AGGCAGCTCACAAGGCAA | TGATCATCGCCTTCAGCCC | 59.48 | 59.85 | 291 | 74 |
100.00%
|
100.00%
|
| Primer 538 | Methylocella silvestris | AAAAGCTTGGCGTTGCGG | AGAACGCTTCCGCTTTGC | 59.97 | 59.05 | 157 | 74 |
100.00%
|
100.00%
|
| Primer 539 | Methylocella silvestris | AAGACCGTCCGCAATCCA | TGCTGATGTCGACGCCAA | 58.93 | 59.66 | 183 | 74 |
100.00%
|
100.00%
|
| Primer 540 | Methylocella silvestris | AGACCGTCCGCAATCCATC | TGCTGATGTCGACGCCAA | 59.86 | 59.66 | 182 | 74 |
100.00%
|
100.00%
|